Here we developed stably transformed Populus tremula × Populus alba hybrid poplar (INRA 717-1B4) containing one-of-six synthetic drought stress-inducible promoters (SDs SD9-1, SD9-2, SD9-3, SD13-1, SD18-1, and SD18-3) identified previously by transient transformation assays. Engineering promoters to control the precise expression of stress resistance genes is a very effective way to address the problem. For sustainable bioenergy crop production, it is critical to generate resistant crops to such stress. Abiotic stresses can cause significant damage to plants.